- Views from Understanding Evolution
- Open Access
Bringing Homologies Into Focus
© Springer Science + Business Media, LLC 2008
- Received: 8 August 2008
- Accepted: 26 August 2008
- Published: 26 September 2008
Anyone who has skimmed a high school biology textbook will be familiar with the iconic examples of homology that seem inseparable from any explanation of the term: the limb structure of four-legged animals, the human tailbone and the more elaborate tail of monkeys, and the remarkable similarities among the embryological development of fish, birds, and humans. These same examples make their way from edition to edition, along with the classic illustration of an analogous structure: the wings of butterflies, birds, and bats. But is that really all there is to say about homologies and analogies? Several articles in this issue discuss these concepts more deeply in the context of eye evolution (Gregory 2008; Oakley and Pankey 2008; Piatigorsky 2008). Homologies and analogies, it seems, are not a black and white issue—especially when it comes to vision.
Convergent evolution. This process produces analogies, as discussed above. Two lineages that begin with different traits evolve a similar characteristic independently of one another, often because both lineages face similar environmental challenges and selective pressures. For example, two distantly related plant lineages might evolve analogous tubular red flowers under selection from hummingbird nectar feeders. Or, as in Fig. 5a, fish lineages that originally had different body patterns might independently evolve analogous vertical stripes, perhaps because of selection for a particular camouflage pattern.
Parallel evolution. In this process, two traits that are already similar (usually because of common ancestry) independently evolve the same set of changes—generally meaning that the same set of underlying genes are involved. This is illustrated in Fig. 5b in which two closely related fish lineages evolve in the same way, resulting in two lineages with a striped body pattern. A real-life example of parallel evolution also involves fish. The ancestral stickleback fish was marine-living and heavily armored by sturdy plates. However, several stickleback lineages invaded new, freshwater environments. Many of these now-freshwater lineages independently evolved the same sort of genetic changes which produce a lightly armored body form. Scientists are not sure why the lightly armored trait was favored in so many different lineages, but it may have involved selection for increased body flexibility (Colosimo et al. 2005).
Evolutionary reversal. In this process, a lineage evolves toward one of its ancestral traits, effectively losing a more recently evolved trait. This is generally thought to involve genetically “reactivating” the ancestral trait (e.g., see Marshall et al. 1994). If a related lineage has retained the ancestral trait, the two lineages will share a similar feature, not because of inheritance from a common ancestor but because of an evolutionary reversal in one lineage. This is illustrated in Fig. 5c in which an originally striped fish lineage loses its stripes and then regains them, producing a species similar to a closely related lineage that never lost its stripes in the first place. A real-life example of evolutionary reversal involves stick insects, which evolved from a winged ancestor but then lost those wings. Stick insects diversified into many different species in their new wingless form. However, a few stick insect lineages seem to have independently “reevolved” wings by reactivating an ancient genetic program that produces wings (Whiting et al. 2003). These newly winged insects now have wings similar to one another and to more distantly related winged insects—insects which never lost their wings in the first place. However, the wings in these different groups are homoplasious since they were not directly inherited from a common ancestor: the winged stick insects went through an intermediary wingless stage.
Eye evolution, in fact, exhibits both convergent and parallel evolution. The similarity in structure between squid and vertebrate eyes (Fig. 3) is a classic example of convergent evolution, since our most recent common ancestor may have borne nothing more complex than a few light-sensitive cells. In a remarkable example of parallel evolution, the same protein (known as zeta-crystallin) appears to have been independently recruited to go to work as part of the lens in two distantly related groups of vertebrates: the llama and guinea pig families (Gonzalez et al. 1995). The zeta-crystallin in the lenses of these relatively remote relatives represents the legacy of parallel evolutionary events, not recent common ancestry. These processes, as well as evolutionary reversal, can produce traits that are strikingly similar—though not inherited directly from a common ancestor.
An examination of eye evolution also highlights another, less familiar aspect of homology. Though we tend to think of homologies in terms of anatomy (e.g., the tetrapod limb, insect wing, or vertebrate eye), any heritable trait—anything that can be directly or indirectly encoded in DNA—can be a homology. Coding and noncoding DNA sequences, the proteins and gene regulation systems encoded by DNA sequences, simple traits (like eye color), components of complex organs (like the lens of an eye), entire complex structures, and even behaviors (like providing parental care to offspring) can be examined to determine whether they are homologous or homoplasious among different lineages.
Which organisms are being compared? The complex lens eye is homologous among humans, lizards, and fish, but the same trait is homoplasious between humans and squid, having evolved independently in vertebrates and mollusks. Simply identifying a trait as homologous or homoplasious is meaningless unless we know which lineages are being considered.
What specific aspect of the trait is being compared? Taking another example from eye evolution, we need to know whether we are considering the entirety of a complex structure (e.g., a lens-type eye), the lens itself, the proteins that make up that lens, the genes that encode those proteins, or the genetic triggers that cause those genes to be turned on in developing eyes. Each aspect of this trait may have a slightly different evolutionary story. Vertebrates, for example, have homologous, lens-based eyes, though some of the crystallin proteins that make up those lenses exhibit homoplasy. Different proteins have been recruited at different points in our evolutionary history to build vertebrate lenses.
Does the function of the trait matter? To make matters even more complex, analyses of homology may also need to consider the function of the trait. Zeta-crystallin, described above, provides a striking example. This molecule is present in modern llamas and guinea pigs—and appears to have been inherited from their common ancestor, making the molecule itself homologous in these two groups. However, in that ancient common ancestor, zeta-crystallin likely performed the job of catalyzing chemical reactions and did not form part of the lens, as it now does in llamas and guinea pigs—making zeta-crystallin homoplasious as a lens component in these two groups (Gonzalez et al. 1995).
Consider the evolutionary history of Pax6, which exemplifies many of these details. Pax6 is a gene that helps control what other genes do. It encodes a special type of protein known as a transcription factor. This protein can invade the nucleus of a cell, bind the DNA there, and turn other genes in that DNA off and on. Such regulatory genes can be enormously powerful, as they can set off a whole cascade of other gene actions.
Through an examination of eye evolution, we have seen that distinguishing between homology and homoplasy can be a tricky and context-dependent business. In fact, these concepts may represent the ends of a continuum more than opposite ideas (Hall 2007). Making even a general differentiation between them depends on specifying the organisms of interest, the aspect of the trait being compared, and even the function of the trait at various points in its history. Homoplasy, especially if it arises through parallel evolution or reversals, may be difficult to detect. Nevertheless, examining these issues for each level of organization of a complex organ (from regulatory genes up to the functioning structure) provides a more accurate and complete understanding of sometimes complex evolutionary histories and helps us understand how sophisticated and highly interdependent organs can evolve from humble beginnings. Especially when it comes to the evolution of eyes, the concepts of homology and homoplasy may not be simplistic, but they are illuminating.
Homologies. We have seen that homologies are simply traits inherited from a common ancestor. However, not all homologies are obvious. If two homologous structures have been adapted for different roles, they may not look very much alike. For example, though they are homologous structures, the chomping front teeth of a beaver look quite different from the tusks of an elephant. Nevertheless, once you know what homologies are, you can find them almost anywhere. This short article from the Understanding Evolution website takes a look at five examples of homology, including structural, genetic, and behavioral examples: http://evolution.berkeley.edu/evolibrary/article/homology_01.
Analogies. As we have seen, analogies are one important category of homoplasy. They often result when two species face a similar problem or challenge and evolution shapes both lineages in similar ways. This short article from the Understanding Evolution website takes a look at six examples of structural analogy: http://evolution.berkeley.edu/evolibrary/article/analogy_01.
Review basic concepts from the field of evo-devo in this advanced tutorial from the Understanding Evolution website: http://evolution.berkeley.edu/evolibrary/article/evodevo_01.
Learn how developmental genetics is reshaping our understanding of evolution in this article from National Geographic: http://ngm.nationalgeographic.com/ngm/0611/feature4/index.html.
Expand your understanding of developmental genetics with this video from Howard Hughes Medical Institute. In lecture 4 of a four-part series entitled From Butterflies to Humans, evolutionary biologist Sean Carroll uses the developmental genetics of insects to explain how old genes can learn new tricks and how this can help us understand human evolution: http://www.hhmi.org/biointeractive/evolution/lectures.html.
An interactive web feature on the evolution of eyes: http://evolution.berkeley.edu/evolibrary/article/eyes_01.
Similarities and differences: Understanding homology and analogy for grades 6–8, http://evolution.berkeley.edu/evolibrary/article/similarity_ms_01.
Similarities and differences: Understanding homology and analogy for grades 9–12, http://evolution.berkeley.edu/evolibrary/article/similarity_hs_01.
Comparison of human and chimp chromosomes. A lesson dealing with homologies at the molecular level from the Evolution and the Nature of Science Institute for grades 9–12. In this lesson, students observe that the banding patterns seen on stained chromosomes from humans and chimpanzees show striking similarities. Possible evolutionary relationships are explored, as are the chromosomes and relationships of other apes: http://www.indiana.edu/~ensiweb/lessons/chromcom.html.
Using trees to understand plants. An article with discussion questions from the Understanding Evolution website for grades 9–12. This research profile follows scientist Chelsea Specht as she uses phylogenies to investigate the pattern of homology and homoplasy in the evolutionary history of tropical plants and their pollinators—and in the process, tries to figure out how to conserve endangered species: http://evolution.berkeley.edu/evolibrary/article/specht_01.
The new shrew that’s not. A short news article with discussion questions, links to lessons, and a podcast from the Understanding Evolution website and the National Evolutionary Synthesis Center for grades 9–12. This news brief from March of 2008 describes scientists’ discovery of a new mammal species, a giant elephant shrew. Though elephant shrews share many analogous traits with regular shrews, genetic homologies suggests that elephant shrews actually sprang from a much older (and perhaps more charismatic) branch of the tree of life—the one belonging to elephants and their relatives: http://evolution.berkeley.edu/evolibrary/news/080301_elephantshrew.
The author wishes to thank T. Ryan Gregory, Todd Oakley, and Judy Scotchmoor for the helpful comments on earlier drafts.
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