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Table 2 PCR conditions and cycling protocol with PerfectTaq DNA polymerase™ (5 Prime)

From: Where Do I Come From? Using Student’s Mitochondrial DNA to Teach About Phylogeny, Molecular Clocks, and Population Genetics

Components

Total volume (μl)

Final concentration

10× PCR Buffer

5

1×

dNTP mix (ten mM each)

1

200 μM

Primers (ten μM) each, Table 1

1 each

0.2 μM each

Water (RNase free)

31.75

–

PerfectTaq enzyme

0.25

1.25 units

DNA

10

~20–200 ηg

Total volume

50

–

  1. Initial denaturation of three min at 94 °C followed by 35 cycles of 30 s at 94 °C, 30 s at 51 °C, and 60 s at 72 °C and a final extension of ten min at 72 °C
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Evolution: Education and Outreach

ISSN: 1936-6434

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