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Table 1 PCR primers and sequencing primers (SEQ) used to amplify the entire human hyper variable region, see Fig. 2

From: Where Do I Come From? Using Student’s Mitochondrial DNA to Teach About Phylogeny, Molecular Clocks, and Population Genetics

Primer name, direction (F or R), and position in relation to human complete mtDNA Sequence (5′-3′)
HVR1 F (15614-15637) AGG CGT CCT TGC CCT ATT ACT ATC
HVR2 R (767-744) CGT GCT TGA TGC TTG TCC CTT TTG
HVR1 SEQ F (15986-16009) CCA TTA GCA CCC AAA GCT AAG ATT
HRV2 SEQ F (104-127) CCG GAG CAC CCT ATG TCG CAG TAT